Immortalization of Primary Cells

Primary cells have a short lifespan before they attain senescence, and it takes time and effort to repeatedly create new cultures from explanted tissues. The uniformity of the experiment’s materials is ensured by the use of the immortalization of primary cells or cell lines. Additionally to having the ability to proliferate for an extended period, immortalised primary cells share the same genome and phenotype as their parental tissue. Furthermore, numerous hTERT-based experiments that immortalise initial cells have produced differentiated cell types with tissue-specific characteristics and differentiation-specific proteins. In addition to these considerations, cell biology research is greatly aided by the simplicity of handling and maintaining cell lines.