SPE CARTRIDGES
RayCure SPE series provides high-quality SPE cartridges for various types of solid phase extraction, including manifold, vacuum as well as automated solid phase extraction equipment, well compatible with RayKol automated solid-phase extraction systems. RayCure SPE cartridges utilize high-quality sorbents to ensure the efficiency of SPE clean up, offers full range of SPE cartridges for various sample matices. Strict quality managment and control ensures high recovery rate of analytes and experiment reproducibility. RayKol provides effective, efficient and economial SPE cartridges with high performance.
RULES TO SELECT RAYCURE SPE CARTRIDGE TYPES
Rule I:
The properties of solid phase packing in SPE cartridges should be match to target compounds.
Rule II:
Take the advantage of differences between analytes of interest and interferences
Rule III:
Consider nature of SPE sample matrices, characteristics of analytes and solubility of used solvents
Users can select RayCure SPE columns depending on properties of desired analytes and sample matrices as following selection guides.
Target compounds
Properties of analytes
Corresponding SPE
Solubility
Polarity/Ionicity
SPE mechanism
Sorbent phase
Water soluble
Compounds with high/low polarity
Non-polar
C18
Polymer
HLB
Ionic compounds
Anion exchange
MCX, WCX
Cation exchange
MAX, WAX
Soluble in organic solvents
Polar compounds
Normal phase bonding
PSA, NH2
Compounds with neutral polarity
Normal phase adsorption
Silica, Florisil
Non-polar compounds
Reversed phase
C18
Based on nature of SPE sample matrices
SPE sample matrices
HOW DOES SPE COLUMN WORK?
SPE is a conventional and efficient extraction technique, it uses the affinity of solutes dissolved or suspended in mobile phase for stationary phase to separate desired compounds from a mixture. And there are two solid phase extraction mechanisms to obtain target analytes from complex samples, to retain desired compounds in stationary phase or retain impurities in solid phase. Depending on the difference of two mechanisms, SPE procedures could be a bit various.
When sample portion passes through SPE column, undesired impurities are discarded with mobile phase, target compounds are retained in stationary phase, then rinse stationary phase with eluents to collect target compounds. It is the most commonly used purification mechanism of solid phase extraction.
In the opposite, to retain impurities in stationary phase, target compounds and few impurities are collected in mobile phase when sample portion passes through SPE column. Rinse the stationary phase which impurities is retained with solvents to obtain rest of retained target compounds. This mechanism is mostly used to eliminate fat-soluble impurities of ion analysis and analysis of multiple pesticides in fruits and vegetables.
