How Are PCR Tubes Sterilized Before Use?

PCR tubes must be sterilized before use to prevent contamination that can interfere with the PCR reaction. There are different methods for sterilizing PCR tubes.

(1)Autoclaving: This is a common method of sterilization that involves subjecting the PCR tubes to high-pressure steam at a temperature of 121°C for 15-20 minutes. This kills any microorganisms on the surface or inside the tubes.

(2)UV sterilization: PCR tubes can also be sterilized using UV light. The tubes are placed in a UV sterilization chamber, where they are exposed to UV light for a specified time, usually about 30 minutes.

(3)Chemical sterilization: PCR tubes can be sterilized by immersing them in a solution containing a sterilizing agent, such as bleach or ethanol. The tubes are then rinsed with sterile water to remove any residual sterilizing agent.

(4)Sterile packaging: PCR tubes can also be purchased pre-sterilized and individually packaged to maintain their sterility until use.

It is important to use sterile techniques when handling PCR tubes to avoid contamination. This includes using sterile gloves, working in a sterile environment, and avoiding contact between the outside of the tube and any non-sterile surfaces. Additionally, it is important to avoid cross-contamination between samples by using separate pipettes and tips for each sample and changing gloves between handling different samples.