Common Errors And Tech Tips For Primary Cell Culture
If you are a cell culture researcher working with primary cell culture, the most difficult thing to accept is watching your cell dishes and flasks getting contaminated or not proliferating even with appropriate incubation at the correct media pH and temperature conditions. Watching those adherent cells not attached to the flask surface and knowing that you have to repeat the whole process of maintaining your cell culture for starting your cell culture-based assays and other experiments, is quite taxing for any researcher.
Not only is this a waste of the time and energy of the researchers involved, but also a lot of money and resources like media and other reagents are wasted in the process and this can be close to a nightmare for a financially tight budget-based research laboratory. Therefore, it is very important to know the tricks and treats of every cell culture process step that are practiced in labs to ensure healthy growth and maintenance of the primary cells.