Mammary Epithelial Cells

For more than two decades, researchers have attempted to develop mammary epithelial cell culture models that resemble human breast cancers in vivo. In order to establish such models, culturing non-cancerous mammary epithelial cells was necessary using a human mammary epithelial cell growth medium. To design an optimum growth medium, researchers prepared a defined medium DFCI-1 to culture mammary epithelial cell and epithelial carcinoma cell lines but there was difficulty in establishing primary carcinoma cell culture.