Nucleic Acid Extraction
NE48/96 is a fully-auto nucleic acid extraction instrument. This nucleic acid extraction device is equipped with a large touch screen and auto-ultraviolet disinfection module. With the rapid heating/cooling and excellent extraction performance, the nucleic acid extraction equipment brings user-friendly operation and many conveniences for the customer. Apart from this, we also produce nucleic acid extraction reagent for detection needs.
What are the different types of nucleic acid extraction?
1: Guanidinium Thiocyanate-Phenol-Chloroform Extraction
2: Cesium Chloride / Ethidium Bromide Gradient Centrifugation
3: Solid-Phase Extraction
4: Magnetic bead-based purification
What is nucleic acid extraction?
Nucleic acid extraction is the first step in the NGS sample prep protocol. Nucleic acids are large biomolecules that are essential to all known forms of life. They are composed of nucleotides, which are monomers made up of three components – a sugar, a phosphate and a nitrogenous base. The two main classes of nucleic acids are DNA and RNA.
What is the importance of DNA extraction?
Extraction of DNA, RNA or total nucleic acids from samples such as blood, cultured cells, microbes or plant and animal tissues is critical to the success of many experiments. Purity and yield of the extracted nucleic acids is key to performance in downstream applications such as PCR and detection.
What is magnetic bead-based purification?
Magnetic separation is now deemed a simple and efficient method used in the purification of nucleic acids. It is a modification of solid-phase extraction. The beads have a negative surface charge and selectively bind to proteins, such as DNA. The binding process may sometimes be assisted by a magnet being applied to the side of the tube as this aggregates the particles near the wall. The remainder of the sample, consisting of cellular debris and unwanted material, can then be poured away. The nucleic acids are removed from the magnetic particles with a buffer and any remaining contaminants are washed away.
What are the advantages of Magnetic bead extraction method？
This method certainly has advantages – it doesn’t need repeated centrifugation, vacuum filtration or column separation, making it time and cost effective. Many commercial kits are available, with some manufacturers even combining magnetic beads with other solid-phase extraction techniques, including the use of silica. Such technologies are helping scientists by enhancing the DNA recovery with only small volumes of sample.